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. 2013 Feb 15;8(2):e56481. doi: 10.1371/journal.pone.0056481

Figure 3. Transforming growth factor-β (TGF-β) activity is associated with Cyr61 gene expression after UUO.

Figure 3

(A) Expression of TGF-β1 transcripts normalized to 18S in the UUO kidneys, measured by Q-PCR. N = 8/time point. (B–C) Active (B) and total (C) TGF-β1 protein concentrations as determined by immunoassay in kidneys of mice receiving sham operation or UUO surgery. N = 4/time point. (D) Representative Western blot of Smad2/3 proteins in the UUO kidneys. (E) The graph summarizes the analysis for renal phosphorylated Smad2/3 normalized to total Smad2/3 after UUO. N = 4/time point. (F) The mice received an intraperitoneal injection of 10 µg/g BW pan-TGF-β monoclonal antibody (1D11) or control antibody (13C4) 2 hours before the UUO surgery and were euthanized the next day. Q-PCR showed that the increased Cyr61 transcripts in the UUO kidneys were attenuated by 1D11 treatment. N = 4/group. The values are the mean+SD. *P<0.05 vs. sham operation kidneys; #P<0.05 vs. 1D11 treated.