Figure 4. Cyr61 expression in cultured renal tubular epithelial cells.
(A) Cultured rat proximal tubular epithelial cells (NRK-52E) were treated with various concentrations of recombinant TGF-β1 for 2 hours. Q-PCR showed Cyr61 upregulation by TGF-β1 at doses of 0.25 ng/mL or greater. N = 4/group. (B) Recombinant Cyr61 was added to NRK-52E cells for 2 or 6 hours. Q-PCR showed increased expression of MCP-1 transcripts by Cyr61 in a dose-dependent manner. N = 4/group. The values are the mean+SD. *P<0.05 vs. control.