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. 2013 Feb 15;8(2):e56481. doi: 10.1371/journal.pone.0056481

Figure 6. Effect of Cyr61 blockade on renal inflammation in the UUO kidneys.

Figure 6

The mice received an intraperitoneal injection of 10 µg/g BW of the control rabbit IgG (filled bar) or anti-Cyr61 antibody (open bar) 2 hours before the UUO surgery and then one dose per day until euthanasia. (A–C) Graphs showing a summary of MCP-1 (A), chemokine (C-C motif) receptor-2 (CCR-2) (B), and F4/80 (C) transcripts normalized to 18S. The data are expressed as the fold differences compared to the sham operation kidneys (gray bar). N = 8/group. (D) Representative F4/80 immunofluorescence photomicrographs (magnification 400×, scale bar = 100 µm) of kidneys from mice at days 4 and 7 after UUO. The bar charts are a summary of the percentage of positive F4/80-stained areas. N = 4/group. (E and F) Quantifying the expression of the profibrogenic macrophage-associated chemokine (C-C motif) ligand 17 (CCL17) and CCL22 transcripts by Q-PCR. Graph showing the ratios of CCL17 to F4/80 (D) and CCL22 to F4/80 (E). All of the values are the mean+SD; *P<0.05 vs. sham operation; #P<0.05 vs. control IgG; N = 8/group.