Figure 3. MADD knockdown in breast cancer cells results in spontaneous apoptosis.
MCF-7 (A), MDA-MB-231(B) and T47D(C) breast cancer cells were transduced for 72 h with indicated viruses, they were then stained with TMRM, harvested and subjected to flow cytometry to determine the percentage of apoptotic cells. *p<0.05;** p<0.01 13L shRNA vs. 16E shRNA. Summarized data from three independent experiments are shown.