Figure 2. Microglial p38β MAPK deficiency failed to reduce LPS-induced TNFα and IL-1β production in microglia/neuron co-culture.

Mouse primary cortical neurons at DIV7 (5×10⁴/well) were co-cultured with microglia (2×10⁴/well) from WT or p38β KO mice, and the cytokine levels of TNFα and IL-1β were measured by ELISA after 72 hr exposure to vehicle or LPS (3 ng/ml). LPS induced a significant increase in TNFα (A) and IL-1β (B) production, and p38β deficiency in microglia did not reduce the production of TNFα and IL-1β. Data represent the mean ± SEM from 2–4 independent experiments. ****p<0.0001 WT-veh vs. WT-LPS; ^###p<0.001, ^####p<0.0001 KO-veh vs. KO-LPS.