Table 2. Thermodynamic quantities of the thermal denaturation of PhaFa.
Probe | Monomer concentration (µM) | t m (°C) | ΔH m (kcal/mol) | ΔG int o(kcal/mol)b,c | ΔG int o (kcal/mol)b,d |
Far-UV CD | 3.8 | 45.5±0.2 | 53.8±2.3 | 25.0±0.3 | 23.5±0.3 |
Far-UV CD | 3.8e | 46.1±0.4 | 44.2±2.8 | 24.0±0.6 | 22.5±0.6 |
Far-UV CD | 38.0 | 50.8±0.3 | 103.6±13.5 | 28.1±1.8 | 26.2±1.8 |
Near-UV CD | 38.0 | 51.5±0.3 | 95.4±12.4 | 27.3±1.6 | 25.4±1.6 |
Heating rate: 1 K min−1 unless otherwise stated
Data calculated for a temperature of 10 °C
Assuming ΔC p = 0 kcal mol−1 K−1
Assuming ΔC p = 0.7 kcal mol−1 K−1
Heating rate: 0.5 K min−1