Table 3. Effect of arginine mutation on EBA-175 RII binding to human erythrocytes.
| Binding of EBA-175 RII mutants to human erythrocytesa | |||
| Normal erythrocytesb | Nm erythrocytesc | ||
| Exp. 1 | Exp. 2 | ||
| DBL-2 | 4 | 1 | 13 |
| EBA-175 RII | 234 | 410 | 9 |
| R566A* | 52 | 112 | 28 |
| R566K* | 248 | 328 | 20 |
a
the rosette number was an average counted from two biological repeats in each experiment.
b
erythrocyte-binding assay was performed with normal erythrocytes in two independent experiments.
c
erythrocyte-binding assay was performed with neuraminidase-treated erythrocytes Nm, neuraminidase.
*
Residue 566 of EBA-175 corresponds to residue 422 of rEBA-175 RII.