Table 2. Primer sequences.
Pappa2 allele genotyping | |
KO proximal | CAGCAAAGGAAATTTGTGCT |
KO distal | ATTCCTCAGCCTCCTCTGAT |
KO exon | CTTCGATGATGGAGACTGCT |
KO neo | GGATTGGGAAGACAATAGCA |
QTL allele genotyping | |
Proximal forward | GGTGTTGTGGGTGAGACATT |
Proximal reverse | AAACATAAAGTAGACATGCTTCCA |
Distal forward | CCTGTCTGACTCACGAAGAAA |
Distal reverse | GGTCATGGTCTCTCTTCATACC |
Cre genotyping | |
Cre forward | GCGGTCTGGCAGTAAAAACTATC |
Cre reverse | GTGAAACAGCATTGCTGTCACTT |
Positive control forward | CTAGGCCACAGAATTGAAAGATCT |
Positive control reverse | GTAGGTGGAAATTCTAGCATCATCC |