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. 2013 Feb 15;8(2):e56257. doi: 10.1371/journal.pone.0056257

Figure 2. Decreased motor neuron survival in E18.5 GAD67-deficient (−/−) hypoglossal and phrenic motor nuclei.

Figure 2

A and B show light micrographs of the hypoglossal (XII) motor nuclei from GAD67 deficient (B) and wild type littermate control (A) at E18.5. C and D show the mean motor neuron numbers ± SEM of hypoglossal motor neurons (C) from GAD67-deficient (−/−) and wild type (+/+) littermates respectively, from embryonic day 13.5 (E13.5) through to E18.5 and phrenic motor neurons numbers from GAD67-deficient (−/−) and wild type (+/+) littermates respectively at E18.5 only (D) (n = 6 per age, *P<0.05, unpaired t test). Scale Bar: A, B, 100 µm.