Figure 2. Nox4 enhances PE-induced cardiac hypertrophy in vivo.
A and B. Expression levels of Nox4 and GAPDH in NTg and Tg-Nox4 mouse hearts subjected to either PE or saline infusion for 2 weeks. The results of the quantitative analysis of Nox4 expression are shown (n=5). C. The effect of PE and overexpression of Nox4 on blood pressure was measured by tail cuff (n=6). D and E. LV myocyte cross-sectional area, as evaluated using wheat germ agglutinin (WGA) staining (n=4). Bar=20 μm. F. Expression of ANF mRNA in NTg and Tg-Nox4 mouse hearts in the presence or absence of PE infusion was evaluated by quantitative RT-PCR (n=6). G. LVW/TL after PE stimulation was determined (n=6). *P<0.05, **P<0.01. N.S.: not significant.