. Author manuscript; available in PMC: 2014 Feb 14.

Published in final edited form as: J Med Chem. 2013 Jan 23;56(3):1074–1083. doi: 10.1021/jm301519z

Table 1.

Kinetic parameters for substrate hydrolysis and inhibition of compound 1

Protease	K_m (mM)	k_cat (min^-1)	k_cat/K_m (min^-1·μM^-1)	Relative k_cat/K_m	K_i (nM)	Relative K_i
PR_WT^a	30±5	190±20	6.5±1.30	1.0	0.50±0.06	1
PR_R8Q	38±2	161±4	4.2±0.25	0.6	1.20±0.08	2
PR_D30N	76±8	48±2	0.6±0.72	0.1	8.9±0.4	18
PR_I50V^a	109±8	68±5	0.6±0.03	0.1	30.9±0.6	60
PR_I54M^a	41±5	300±40	7.3±0.80	1.1	3.5±0.2	7
PR_V82A^b	29±3	512±26	17.7±2.00	2.7	3.2±0.2	6

K_m and k_cat values previously reported in ²²

K_m and k_cat values previously reported in ⁴¹