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. Author manuscript; available in PMC: 2014 Feb 11.
Published in final edited form as: Dev Cell. 2013 Jan 24;24(3):310–323. doi: 10.1016/j.devcel.2012.12.015

Figure 2. Double knockout ESCs remain pluripotent in teratoma and chimera assays.

Figure 2

(A) Representative images of each germ layer from H&E stained sections of teratomas generated from ESCs of indicated genotypes. (B) High magnification images of H&E stained teratoma sections showing hemorrhage and trophoblast-like cells (arrowheads) in tumors derived from DKO and Tet1 deficient ESCs. (C) E14.5 chimeric embryos generated by injecting FUW-td-Tomato transduced ESCs of indicated genotypes into wild type blastocysts. Notice exencephaly and weaker td-tomato signal in DKO ESCs chimeras. (D) Summary table for all chimera injections and defects of DKO chimeric embryos.