Table 1.

Methylation analysis of imprinted genes in DKO mice (see also Table S1)

A: Analysis of mice from progeny of DKO male × or WT female
Mouse Phenotype	Aberrant Mest Methylation	Aberrant Peg3 Methylation	Aberrant Methylation of both genes
Dead or abnormal mouse	2 (out of 8)	0 (out of 8)	0
Viable and normal mouse	1 (out of 8)	2 (out of 8)	0
Control (Progeny of WT male × WT or Dhet female)	0 (out of 3)	0 (out of 3)	0

B: Analysis of mice from progeny of WT male × DKO female
Mouse Phenotype	Aberrant H19 Methylation	Aberrant Igf2r Methylation	Aberrant Methylation of both genes
Dead or abnormal mouse	8 (out of 10)	3 (out of 8)	3
Viable and normal mouse	2 (out of 3)	0 (out of 3)	0
Control (Progeny of WT or DKO male × WT female)	0 (out of 2)	0 (out of 1)	0

C: Analysis of sperm from DKO mouse
Genotype of sperm source	% Mest Methylation	% Peg3 Methylation	% H19 Methylation
DKO	10.5	1.1	89
WT	1.2	1.1	94