The release profiles were determined in PBS solution at room temperature using Griess reagent to measure the nitrite concentration produced from the released NO. Absorbance values at 550 nm were determined using a Molecular Devices Plate Reader.
a) NO release profiles of NO-donors, A6’–A10’, b) NO release profiles of NO-donors, B6’–B10’. A fluorescent probe DAF-FM was used as an alternate probe to detect NO and/or N2O3. Fluorescent signals were detected using the same micro-plate reader at 495/515 (ex/em) c) NO release profiles of A7’ and B7’ determined using DAF-FM.