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. Author manuscript; available in PMC: 2014 Mar 1.
Published in final edited form as: Bioorg Med Chem. 2013 Jan 9;21(5):1123–1135. doi: 10.1016/j.bmc.2012.12.043

Figure 3.

Figure 3

a) Analyses of nitrite production from NO-donor, A6’ in the presence and the absence of C-PTIO in 1X PBS. b) Analyses of nitrite production from NO-donor, A6’ in the presence and the absence of C-PTIO in supplemented SMC culture medium. Aliquots were removed every 24 h, treated with Greiss reagent and absorbances measured at 550 nm (data displayed in 3a). Data for 3b were obtained from absorbances measured at 550 nm with a background subtraction at 630 nm. C-PTIO rapidly captures released NO radical species before a fraction can escape and converts it to nitrite (detected by Griess reagent). Consequently, the presence of C-PTIO results in significantly higher measured nitrite concentrations (higher absorbance values) than in its absence at any point in time during the span of the experiment (5 days).