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. Author manuscript; available in PMC: 2014 Mar 1.
Published in final edited form as: Bioorg Med Chem. 2013 Jan 9;21(5):1123–1135. doi: 10.1016/j.bmc.2012.12.043

Figure 6.

Figure 6

Determination of cytotoxic effects by secondary amines on HASMC cultures using a LIVE/DEAD assay. Two distinct fluorescent measurements at em 495/ex 515 and em 495/ex 635 provided data points for calculating reported percentages of LIVE and DEAD cells respectively. (*) Indicates a statistically significant difference (p < 0.05) between the given compound/concentration combination and the vehicle alone. The concurrent decrease in LIVE cells and increase in DEAD cells exhibited by the negative control suggests 1% DMSO induces cell death. Therefore the use of DMSO as the vehicle accounts for the percentage of DEAD cells present in all tested samples. The graphical data represents the mean ± SE of triplicate determinations, and reveal the percentages of LIVE and DEAD cells resulting from exposure to a) A6–A10 and b) B6–B10.