Figure 1.
GRP78 is required for adipogenesis from embryonic fibroblasts. A) Grp78+/+ and Grp78f/f MEFs were infected with adenovirus-Cre (Ad-Cre) and induced to differentiate into adipocytes at d 0. Western blots were performed to detect the levels of GRP78 and XBP-1s, with β-actin serving as loading control. B) Oil Red O staining of MEFs treated in A on d 0 and 16. C) Western blot detection of GRP7 and β-actin levels in Grp78f/f MEFs either noninfected or infected with Ad-Cre. D) Oil Red O staining of MEFs treated in C on the days indicated. E) Real-time-PCR analysis of adipocyte marker genes aP2, PPAR-γ2, and SREBP-1c in MEFs treated in C. Expression levels of each gene were normalized to the levels of 18S rRNA. The experiment was repeated twice, in duplicate each time. Data are presented as means ± se. Scale bars = 50 μm. *P < 0.05; **P < 0.01.