Figure 5.

Ca²⁺ and Na⁺ concentrations before and during halothane exposure in the vastus lateralis muscle in vivo. Intracellular Ca²⁺ determinations (A) or Na⁺ determinations (B) in vastus lateralis muscle were done using ion-selective microelectrodes and performed as described in Materials and Methods. The intracellular Ca²⁺ and Na⁺ concentrations in the absence (shaded columns) or in the presence (solid columns) of 1.5% halothane are shown. Experiments were done in the presence of BTP2 (5 μM), GsMTx-4 (5 μM), Gd³⁺ (25 μM), or dantrolene (40 μM). Means ± se for each condition are shown. Measurements were done in ≥5 mice/group***P < 0.001 vs. corresponding control treatment group; ^&P < 0.05, ^&&&P < 0.001 vs. corresponding WT group.