Figure 4.

Aberrant lymphocyte trafficking in Spns2^−/− mice. Lymphocytes of wild-type (WT) and Spns2^−/− mice were analyzed by flow cytometry. A) Number of circulating CD3⁺ T cells that are CD4⁺, CD8⁺, or CD62L⁺; B220⁺ B cells; Gr-1⁺ granulocytes; and CD11b⁺/c⁺ dendritic cells. B, C) Total number of CD3⁺ T cells that are CD4⁺, CD8⁺, or CD62L⁺ and B220⁺ B cells in lymph nodes (B) and spleen (C). D) Flow cytometry analysis of wild-type and Spns2^−/− thymocytes. Single-positive CD4⁺ and CD8⁺ T cells were gated for the maturation markers CD69 and CD62L. Representative flow cytometry plots (top panels) and total number of CD4⁺CD62L⁺CD69⁻ and CD4⁺CD62L⁺CD69⁻ T cells (bottom panels) are shown. *P < 0.01.