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Impaired T-cell egress from lymph nodes in Spns2-deficient mice. Lymph nodes from wild-type (A, B) and Spns2−/− mice (C, D) were immunostained to visualize LECs with LYVE-1 (green), T cells with CD90.2 (red), and nuclei with Hoechst (blue). Confocal fluorescent images at ×40 (A, C) and ×95 (B, D). Scale bars = 100 μm.
