Figure 2.

Alignment and identification of conserved residues and TM domains in stramenopile and choanoflagellate SITs. The black line is the TMPred SdSIT topology prediction. The orange line is the diatom SIT TMD prediction [30]. Red boxes indicate conserved motifs of three or more residues. The double blue circles mark the ‘CMLD’ motif in stramenopiles. Conserved charged or hydroxylated residues are noted as per [13,30]; purple arrows, negatively charged; red arrows, positively charged; green arrows, hydroxyl-containing. Oo, Ochromonas ovalis (chrysophyte, non-diatom stramenopile); Cf, Cylindrotheca fusiformis (pennate diatom); Pt, Phaeodactylum tricornutum (pennate diatom); Tp, Thalassiosira pseudonana (centric diatom); Sa, Synedra acus (pennate diatom).