Figure 3.

Phylogenetic tree demonstrates that choanoflagellate SITs are monophyletic within the stramenopile SITs. The tree was produced using PhyML ML analysis with the LG + Γ model from an alignment of 769 amino acid residues. The tree produced using RaxML and the WAG model had identical topology. Numbers at nodes are a percentage of 100 bootstrap replicates (PhyML bootstrap value/RaxML bootstrap value). Blue, pennate diatom; green, centric diatom; brown, non-diatom stramenopile; red, choanoflagellate. Dash (−/−) indicates a bootstrap value less than or equal to 70%. Scale bar indicates the average number of amino acid substitutions per site.