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. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: J Thromb Haemost. 2012 Jun;10(6):1165–1171. doi: 10.1111/j.1538-7836.2012.04712.x

Fig. 4.

Fig. 4

Inhibition of factor VII-activating protease (FSAP) activity by tissue factor pathway inhibitor (TFPI), TFPI-160, TFPI-K1M, and TFPI-K2M. Increasing concentrations of TFPI, TFPI-160 (A), TFPI-K1M or TFPI-K2M (B) were added to the chromogenic substrate S2288 (1 mm). Purified two-chain (tcFSAP) (10 nm) was added, and the absorbance at 405 nm was measured. Data are plotted as Vi/Vo against the inhibitor concentration. Vi and Vo indicate the velocities of substrate hydrolysis by 10 nm tcFSAP in the presence and absence of inhibitor, respectively. Results are given as mean ± standard error of the mean (n = 3).