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. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: Mol Genet Metab. 2012 Mar 23;106(2):189–195. doi: 10.1016/j.ymgme.2012.03.009

Fig. 1.

Fig. 1

GAA primary amino acid sequence and MHC II epitope prediction. A. Primary amino acid sequence of the lysosomal protein, acid α-glucosidase. Yellow highlights represent sequences, which were subsequently identified to contain CD4+ T cell epitopes: peptide 19 (HSRAPSPLYSVE), peptide 29 (VLQPSPALSWRS), and peptide 83 (IFLGPEPKSVVQ). B. Prediction of the immunodminant I-Ab-restricted epitope by NET MHC II, a T cell epitope prediction server of the immune epitope database analysis source. Protein was loaded in the Fasta format and sequence IFLGPEPKSVVQ highlighted in yellow was identified as the strongest binder based on its threshold values (strong binder threshold 50.00, weak binder threshold 500.00).