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. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: Mol Genet Metab. 2012 Mar 23;106(2):189–195. doi: 10.1016/j.ymgme.2012.03.009

Fig. 4.

Fig. 4

Confirmation that identified peptides contain CD4+ T cell epitopes. A. Representative examples of well images from IFN-γ ELISpot assays after stimulation of CD4+ cell-depleted splenocytes with peptides 19, 29, 83, rhGAA protein, SEB or solvent control. GAA−/− 129SVE had been immunized with rhGAA/CFA. B. Average frequencies of IFN-γ spot forming units (± SD, triplicate) after CD4+ depletion and stimulation with indicated peptides or controls. C. Representative examples of well images from IFN-γ ELISpot assays after stimulation of CD8+ cell-depleted splenocytes. D. Average frequencies of IFN-γ spot forming units (± SD, triplicate) after CD8+ depletion and stimulation with indicated peptides or controls.