Figure 10. An activity-based probe and SDS-PAGE were used to detect residual DPAP1 activity in Plasmodium falciparum parasites treated for 0.5–6 h with test compounds at the indicated concentrations.

DPAP1 is inhibited in a time dependent fashion by fragmenting hybrid 21, an observation that is consistent with the slow release of active DPAP1 inhibitor 20 from 21, as desired.