Figure 2. Overexpression of TTP in HNSCC cell lines decreases cytokine secretion.
UM-SCC-11A, UM-SCC-17B and OSCC3 cells (A, B and C respectively) were transduced with adenovirus-5 containing β-gal (control) or TTP. Cell lysates and conditioned media were harvested. Cell lysates were electrophoresed and immunoblotted with TTP antibody. Conditioned media was used to quantify IL-6, VEGF, and PGE2 secretion by ELISA. All experiments were performed in triplicate (p<0.001, Standard error bars are present in all graphs).