Figure 3. TTP knockdown induces cytokine secretion in HNSCC cells.
UM-SCC-1 (A), UM-SCC-22B (B) and UM-SCC-81B (C) were transfected with siRNA against TTP or non-target siRNA (nt). Whole cell lysates were electrophoresed and blotted with TTP antibody. Actin was used as loading control. Conditioned media was assayed in triplicate for IL-6, VEGF, and PGE2 by ELISA. All experiments were performed in triplicate. Data are representative of the mean and standard deviation of three replicates within an experiment. (*p<0.05).