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. Author manuscript; available in PMC: 2014 Feb 15.
Published in final edited form as: J Immunol. 2013 Jan 16;190(4):1416–1423. doi: 10.4049/jimmunol.1201620

Figure 5. IL-7 and IL-15 regulate T cell responsiveness by augmenting a positive feedback loop involving SOS.

Figure 5

(a) T cells conditioned with increasing concentrations of IL-7 or IL-15 for 24 h were tested for TCR-induced ERK phosphorylation. Increased cytokine concentrations induced a heteroclitic pERK response consistent with the activation of a feedback loop. (b) Active RAS after cytokine priming was assessed using the Millipore active RAS ELISA kit. Results are shown as mean ± SD of triplicate wells. (c and d) T cells were transfected with siRNA specific for SOS-1 and SOS-2 or control siRNA. Seventy-two hours after transfection, T cells were exposed to IL-15 for 24 h, washed and activated by CD3 crosslinking. Phosphorylation of ERK was measured using PhosFlow (c) and Western blot (d). Each data set is representative of three independent experiments.