Table 1.
Gene | Primer sequence | Accession number | Expected size (bp) |
E-cadherin | 5′-CCCGGGACAACGTTTATTAC-3′ | NM_004360.3 | 190 |
5′-GCTGGCTCAAGTCAAAGTCC-3′ | |||
Vimentin | 5′-AAAGTGTGGCTGCCAAGAAC-3′ | NM_003380.2 | 200 |
5′-AGCCTCAGAGAGGTCAGCAA-3′ | |||
α-SMA | 5′-GCGCAAATACTCGGTGTGGA-3′ | NM_001141945.1 | 170 |
5′-CCCCCCCATTGAGAAGATTC-3′ | |||
Snail | 5′-TGGTTGCTTCAAGGACACAT-3′ | NM_003068.3 | 141 |
5′-GTTGCAGTGAGGGCAAGAA-3′ | |||
SOCS3 | 5′-CAGGAATGTAGCAGCGATGGAA-3′ | NM_003955.3 | 125 |
5′-CCTGTCCAGCCCAATACCTGA-3′ | |||
β-actin | 5′-ATCGTGCGTGACATTAAGGAGAAG-3′ | NM_001101 | 179 |
5′-AGGAAGGAAGGCTGGAAGAGTG -3′ |
α-SMA: α-smooth muscle actin; SOCS3: Suppressor of cytokine signaling 3.