Fig. 4.

Deuterium MAS NMR spectra of the TpoR TM domain. Deuterium MAS NMR spectra were obtained of the wild-type Tpo receptor TM peptide (residues 488–529) and of peptides with mutations at S505 and in RWQFP insert: (A) wild-type, (B) RWQFP deletion mutant, (C) S505N, (D) W515K, (E) R514W, W515K, and (F) W515K, Q516W double mutation. The peptides were deuterium labeled at Leu512 and reconstituted into DMPC:DMPG bilayers at a peptide/lipid molar ratio of 1:50. Each experiment was repeated using separate reconstitutions.