Fig. 7.

Role of TseL and VasX in killing eukaryotic cells. (A) Determination of the minimal number of amoebae required to form a plaque on lawns of different strains of V. cholerae. (B) Western blot analysis of VgrG1 in the tseL and vasX double mutant. (C) Determination of the level of actin cross-linking in J774 cells exposed to various V. cholerae mutants. All strains are defective in RtxA toxin, HlyA hemolysin, and HapA hemagglutinin/protease to eliminate background toxicity and actin cross-linking that are not associated with the T6SS system of V52 (11).