Table 1.

Sequencing Primers and PCR conditions.

Region	Primer name	Sequence (5′-3′)	Tm (°C)	Product size (bp)
5′-upstream	5′_F	CAG AAG GGA TGA CAT GCA GA	60	767
	5′_R	GGC TAT GTG CAT GGA GCT TT
Exon 1	E1_F	GAT TCT TTG CCA ACT TCC AAG	60	363
	E1_R	GAT TAG CAC CCC AAG TCC AA
Exon 2	E2_F	GCA GGA AAG GAC CTG ATG AA	60	323
	E2_R	AAG CTG CTC TTG GCA ATC AT
Exon 3	E3_F	TGA CGT CTC CAA ATA AGC TTC C	60	301
	E3_R	AGG TTG ACA AGA GCT TCA TCC
Exon 4	E4_F	AGG ATC AAA GTC TGG CTT CC	60	305
	E4_R	GGA TGA AGT GGA CGT GGA AC
Exon 5	E5_F	TCT AGC ATA GGG CCC ATC AC	60	352
	E5_R	CA GTG GAC TAC CCC TTG GAA
Exon 6	E6_F	CCA AGG GGT AGT CCA CTG AA	55	362
	E6_R	GGA ATA ACC CAA CAG CAG GA
Exon 7	E7_F	TGG AGT GTG ATA GAA GGT GA	55	516
	E7_R	TTG TGA CAG GGG GCT GAT AG
Exon 8	E8_F	TGC TCC AGG TAA ATT TTG CAC	60	369
	E8_R	CAA ACC CCA CTT TCT GCA TT
Exon 9	E9_F	CAT CCT GCT TTC CAA GGA	60	418
	E9_R	CCT GCA TGC CTC TAG AAA GTG
Exon 10	E10_F	TGA TGC CCT ACA TTG ATC TGA	60	391
	E10_R	CTG CCA GTA GCA ACC ATT TG
Exon 11	E11_F	CCC GAA TGC TTC CCA CCT	60	506
	E11_R	GGC AGA ATA TGC TTG AAC CAG
Exon 12	E12_F	GAC TGA AAG CTC CTA TAG TGT C	60	598
	E12_R	CCA TGC TAA TCT ACA TGG GCT
Exon 13	E13_F	GCC ATC ATA CCT AAT AAT CTG G	60 Xhosa	988
	E13_R	AT GTG CAG GAA AGC ATC TGA	55 Others

5′, 5′-upstream; E, exon; F, forward; R, reverse.