. 2013 Feb 18;4:17. doi: 10.3389/fgene.2013.00017

Table 2.

PCR_RFLP primers, PCR conditions, and genotyping assays.

Position in gene	Allele	rs number	Genotyping assay	Primer name	Sequence (5′-3′)	T_m (°C)	Product size (bp)
−392 A>G	CYP3A4^*1B	rs2740574	MboII	5′_F_m1	GGA CAG CCA TAG AGA CAA GGG gA	55	350
−392 A>G	CYP3A4^*1B	rs2740574	MboII	5′_R	GGC TAT GTG CAT GGA GCT TT	55	350
−292 T>G		Novel	RsaI	5′_F	CAG AAG GGA TGA CAT GCA GA	55	575
−292 T>G		Novel	RsaI	5′_R_m	CCT CCT TTG AGT TCA TAT TCT ATG AGG TAT CgT
−215 T>A		rs144721069	Hpy188III	5′_F_m2	TGT GTG TGT GAT TCT TTG CCA ACT TCt cAG G	55	181
−215 T>A		rs144721069	Hpy188III	5′_R	GGC TAT GTG CAT GGA GCT TT	55	181
3847 A>G		Novel	NlaIII	E2_F_m	GCA GGA AAG GAC CTG ATG AAC AcA T	55	323
3847 A>G		Novel	E2_R	AAG CTG CTC TTG GCA ATC AT		55	323
5916 T>C		rs12721625	BseNI	E3_F	TGA CGT CTC CAA ATA AGC TTC C	60	301
5916 T>C		rs12721625	BseNI	E3_R	AGG TTG ACA AGA GCT TCA TCC	60	301
13969 G>A		Novel	ApoI	E5_F	TCT AGC ATA GGG CCC ATC AC	60	352
13969 G>A		Novel	ApoI	E5_R	CA GTG GAC TAC CCC TTG GAA
14268 C>T	CYP3A4^*23	rs57409622	AciI/Sequencing	E6_F_m/E6_F	GAT GTG TTG GTG AGA AAT CTG AGa C	55	362/154
14268 C>T	CYP3A4^*23	rs57409622	AciI/Sequencing	E6_R	GGA ATA ACC CAA CAG CAG GA	55	362/154
14269 G>A	CYP3A4^*15	rs4986907	AciI/Sequencing	E6_F_m/E6_F	GAT GTG TTG GTG AGA AAT CTG AGa C	55	362/154
14269 G>A	CYP3A4^*15	rs4986907	AciI/Sequencing	E6_R	GGA ATA ACC CAA CAG CAG GA	55	362/154
15619 A>G		rs111768354	Sequencing
15628 C>T		rs4987159	Sequencing
15649 A>T	CYP3A4^*24	rs113667357	Sequencing
15753 T>G		rs2687116	Sequencing
15783 T>C		rs4987160	Sequencing
15804 T>G		rs28988584	Sequencing
15837 T>A		rs12721622	Sequencing
17829 T>C		Novel	PsiI	E9_F	CAT CCT GCT TTC CAA GGA	60	418
17829 T>C		Novel	PsiI	E9_R	CCT GCA TGC CTC TAG AAA GTG	60	418
20230 G>A	CYP3A4^*1G	rs2242480	RsaI	E10_F	TGA TGC CCT ACA TTG ATC TGA	60	391
20230 G>A	CYP3A4^*1G	rs2242480	RsaI	E10_R	CTG CCA GTA GCA ACC ATT TG	60	391
20309 G>C		rs4986911	HindIII	E10_F	TGA TGC CCT ACA TTG ATC TGA	55	327
20309 G>C		rs4986911	HindIII	E10_R_m	CAG TGA AAG AAT CAG TGA TTA aG	55	327
20327 T>C		rs34738177	MboII	E10_F	TGA TGC CCT ACA TTG ATC TGA	60	391
20327 T>C		rs34738177	MboII	E10_R	CTG CCA GTA GCA ACC ATT TG	60	391
21896 C>T	CYP3A4^*12	rs12721629	PstI	E11_F	CCC GAA TGC TTC CCA CCT	55	334
21896 C>T	CYP3A4^*12	rs12721629	PstI	E11_R_m	CAT CTT TTT TGC AGA CCC TCT gcA	55	334
23081 C>T		rs12721620	HpyCH4IV	E12_F	GAC TGA AAG CTC CTA TAG TGT C	60	598
23081 C>T		rs12721620	HpyCH4IV	E12_R	CCA TGC TAA TCT ACA TGG GCT	60	598
25721 A>G		rs3735451	BsaJI	E13_F	GCC ATC ATA CCT AAT AAT CTG G	60 Xhosa	988
25721 A>G		rs3735451	BsaJI	E13_R	AT GTG CAG GAA AGC ATC TGA	55 Others	988

5′, 5′-upstream; E, exon; F, forward; R, reverse; m, mutagenic primer; lowercase and bold letters, mutagenic bases for the introduction of restriction enzyme recognition sites.