Table 3.

The primers for amplifying the CLCNKB gene in the study

	Forward primer	Reverse primer	Length(bp)	Tm(°C)
Exon1	GGACATTCTAAGTGTTCGCCATAA	CCCAGAAGAAGATCCCACCAG	269	59
Exon2	ACACCCTCAGTGACGGAAAC	TGCCACCCAGGAGACTTT	446	59
Exon3	CCACTGTCACCTCCCACAAAT	GCCCAGGATCACAGAGCAAG	481	61
Exon4	TGGGTGCCTCCCTGATAC	TCCTGGGCCTTTGTCTGT	265	61
Exon5	ATCTGGCGAGATCGTAATG	GGTAGGGTGGTTGGGATG	276	62
Exon6, 7	CAAAAGCCATCTGAGGACG	GAGGGGAGGAGCTTGAGG	424	65
Exon8	TGACTTGATTGGCGGTGCTA	CCCTCCATCCTCCCAGAAG	646	59
Exon9, 10	TTAGTCTGGGACTTGAGTTTGGG	GGTTGGGAGTACGGTGAGGG	431	59
Exon11	TTGGGATGTGGGAAAGGG	AAAGGAGGCAACAAATAGGG	194	59
Exon12	GGGAAGTGGCAGAGGAGGA	CAGGAAATGTGGGTGGAAGG	393	62
Exon13, 14	TGTCCTGTCCTCCCTTGT	CCCTGACCCACTCACCT	430	62
Exon15	ACTCCCTCGTGGCTCCTGT	AGCTACGGTGGCGTTTCTTT	516	61
Exon16	TAGGAGTCCCTCATTCCAG	CACGGTTGGTTGCTAAGTC	403	60
Exon17, 18	CCCCATAGGAACACCAGAACA	GCAGGAAGCAGCATAAGACG	552	60
Exon19	ACCTTCTACCCTCCAGTGTTT	TCATCAGTATCCTCTGAATCCC	259	59
Exon20	CCCTCACAACCTCCTCTACATC	TGACCACGGGAAACTCACTCT	588	60