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. Author manuscript; available in PMC: 2013 Feb 18.
Published in final edited form as: J Invest Dermatol. 2012 Aug 16;133(1):181–190. doi: 10.1038/jid.2012.256

Figure 4. Keratin-free cells adhere much faster.

Figure 4

Immunofluorescence analysis of wildtype and keratin-free keratinocytes in the course of adhesion process after 30 min, 1h, 2h and 3h are shown. Therefore, cells were immunolabeled for hemidesmosomal proteins plectin, β4-integrin, the extracellular ligand laminin-332 and keratin 5. (a) Hemidesmosomal structures visualized by plectin and β4-integrin can be seen after 3h in wildtype keratinocytes. In contrast, keratin-free cells are already adherent after 1h. Moreover, plectin and β4-integrin co-localization is missing in keratin-free keratinocytes. (b) Quantitative analysis of non-adherent cells on specific time points. Bar, 10 μm.