Table 1.

Sequences of the primer set designed according to the criteria of primer design used in conformation sensitive capillary electrophoresis (CSCE)^a

Amplicon	Forward primer sequence 5′-3′	Reverse primer sequence 5′-3′	Size bp
3	CGAACATGTAAGTGCCGCTGTCA	AGAACACAGCACCATCTTTGCACAA	380
4	GTAGTGCCAAAACAGCATTAGGTTTGC	GCACTGCCATGGTTCATACAGTAACAT	457
5	TTCCATTAAGTTTTGTTGCAAC	CTGCAATGAGAGAATGGACTAATACAC	435
6	TGAACCCAAGTGTTTGACCTCTTTTCC	CCTTTCTCTTATTTGTGTGTATATGTG	432
7	AACTTTTCCTGTAACAGTATCATCG	AATACAGGTTCTAAGTAATTTTCAACC	429
8	GCACTTTGGCGTTTCTCCTGTGA	GACGTTACCCAAAAGAGAGTTTTCCCT	460
9	GGGAGGAGGTAGGAGGATAC	CACATATAGAAACATGGCCCACACACA	456
10	CTGTGTGTGGGCCATGTTTCTATATGT	TTCCCAAAAGGCAATTAACTGCCTGAA	409
11	CTGCATTTCTCCATCTGCTCTAGCAA	ATTTAAGAAATGTACTGAACTCACATG	440
12	CATCCTGCTAGATTTACTCAAAAAGCC	ACCAATAGACTAATGGGGAAGAAAATC	432
13	TTAAAAACCAGTGTTTCCTTGAAG	AATGCTTGTGTCCAACTAGC	381
14	TATGTCAAATCATGCCTCCTTTTGTC	GAAATGAGGTATCTTACCCCAAAGTAG	428
15	CCATTTCTCCAGTTAAGTTATGGG	TGGGTATGATTGTGACCAAGTGTCAGA	445
16	GGTCACAATCATACCCATATACTTC	AAACCACTGAAATCTGGACAAAGG	442
17	CTATGGCATGTTGTGCTAGTGGAAGT	TCCACATACACCTGAGAAGCAGAAAGA	433
18	AGGAGCTTGGAGCCAAGGGTTT	CCATCATACCTGGCCAAGTTACCAAA	447
19	GATTTGAAACCACTTTAGCCTTCC	TGTGGCAACTCCAGCTTGTTTAAC	340
20	TGGGACTCTCATCTTACTACTGTG	GCATGTGGATCAAGACTAACTCTG	340
22	TTGAAAACTTGTCTCCAGGAAGTG	TGGACCGTACTTTGAGTAGCAAGGAT	402
22	GAATGCTGAGTTCCTAAAACATACAC	TGCAACCCAAGTAGGCATACTCTGA	366
23	TTGTGGACTGGGTAGCCCTTGT	GAAGGAAGGAGGAAAATGGTTCAGGTT	397
24	CCTCCTTCCTTCATCATCTTTCAG	CTATCCACCTACAAGCCTTTTCAG	413
25	TGGGTGAAATGAAAGCAGTTTTG	AAAATCTTGAGTAGCATTACAAGC	458
26	ACCCCAGGTTTAGAGTAACTGTTC	CTACCTAAAGAAAATACAGCTCCC	323
27	CAAAAGTGACTGGTTTTTGTCTTC	GGTGCCAAATCAATACTGACATTTG	440
28	CTGGCCTCACCCCAATTCCTATTTC	ATTATATCGTGAGGTTTGGCACAC	352
29	CAAACTGAGCTTTAGAGTGGTTGTCCT	AGATGAAGGGAAGAAGGCAGGGAAAT	398
30	TTCATTACAATTGTTTACCGAATGCCC	GGGTTTTCCGATATTAGCTGATAG	301
31	CACATCTCAATTCAGACTGGCCACAT	AACAAATGGGAATAAGGAACTAAGC	441
32	GGCTTTCCTAACTTCTACGGCCAAAA	AGATGGCATCTCCTTTTGTTGCCC	407
33	TGCCGAGCTTATTCTGTAGAAGAC	AGGCCACAGCCACTCCTAAAAAAG	333
34	TCACCAAGGACCTGTAAGAATTTC	CCTAGGGCATACAGAAATCAATTC	350
35	CACTAGAAGGCAAAAATCACCAGGTCT	AACTTGAGCCTGTGCATATAAGGCATT	294

^aPrimer and amplicon criteria: Optimal primer length 20bp (minimum 18bp; maximum 27bp), optimal annealing temperature 58°C (minimum 52°C; maximum 64°C), optimal GC% 55 (maximum 70), optimal amplicon size 400bp (minimum 200bp; maximum 464bp), maximal amplicon GC% 73. A PT1 tail was added to every forward primer sequence (5′-3′; TGTAAAACGACGGCCAGT), and a PT2 tail was added to every reverse primer sequence (5′-3′; CAGGAAACAGCTATGACC)