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Ligation of thiothreonyl-pdCpA derivatives to tRNACUA-COH. The efficiency of ligation was monitored by 8% denaturing PAGE, pH 5.2, and the gel was stained with methylene blue. Lane 1, abbreviated tRNACUA (1 μg) without pdCpA; lane 2, ligation of DMT-thiothreonyl-pdCpA with tRNACUA (1 μg); lane 3, ligation of DMT-allo-thiothreonyl-pdCpA with tRNACUA (1 μg).
