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. Author manuscript; available in PMC: 2013 Apr 15.
Published in final edited form as: Bioorg Med Chem. 2012 Feb 15;20(8):2679–2689. doi: 10.1016/j.bmc.2012.02.024

Figure 5.

Figure 5

Coupling of the NVOC-protected thiothreonyl-tRNA analogues with CPM after DMT group removal. The fluorescence was monitored at ~470 nm following irradiation at 365 nm. Sample 1, CPM treated suppressor tRNA (3 μg/μL); sample 2, CPM treated DMT-thiothreonyl-tRNA (3 μg/μL); sample 3, CPM coupled thiothreonyl-tRNA (3 μg/μL); sample 4, CPM treated DMT-allo-thiothreonyl-tRNA (3 μg/μL); sample 5, CPM coupled allo-thiothreonyl-tRNA (3 μg/μL).