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. Author manuscript; available in PMC: 2013 Apr 15.
Published in final edited form as: Bioorg Med Chem. 2012 Feb 15;20(8):2679–2689. doi: 10.1016/j.bmc.2012.02.024

Figure 6.

Figure 6

In vitro incorporation of thiothreonine derivatives into csDHFR at position 10. The samples were analyzed by 15% SDS-PAGE at 100 V for 2 h. Phosphorimager analysis was performed using an Amersham Biosciences Storm 820 equipped with ImageQuant version 5.2 software from Molecular Dynamics. Lane 1, wild-type csDHFR; lane 2, incorporation in the absence of any amino acid; lane 3, incorporation of DMT-thiothreonine; lane 4, incorporation of DMT-allo-thiothreonine; lane 5, incorporation of thiothreonine; lane 6, incorporation of allo-thiothreonine; lane 7, incorporation of CPM-allo-thiothreonine.