Figure 3. .

In vivo knockdown studies of MRP8 and MRP14. Clinical scores (A) showed statistically significant differences in siMRP8/14- and siCTL-treated corneas at 3 and 5 days p.i. (P < 0.01 for time points). The horizontal lines among the open and filled dots represent the means of indicated clinical scores. Three individual experiments were performed, each with 10 animals per group each time. Data were generated from one representative experiment. (B) Slit-lamp photographs of PA-infected eyes at 5 days p.i. displayed reduced disease severity in siMRP8/14-treated compared to siCTL-treated mice. Most of the siCTL-treated corneas were perforated at 5 days p.i. (black arrow highlights the perforation). PCR (C) and Western blot (D) data confirmed the effect of silencing MRP8 and MRP14 in the B6 corneas at 5 days p.i. MRP8/14 silencing decreased PMN infiltration as detected by MPO activity (E) and increased viable bacterial numbers (F) at 5 days p.i. Data are the mean ± SEM and represent three individual experiments, each with five animals per group per time per assay.