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MRP8/14 silencing reduced pro-inflammatory cytokine production in vitro. Human monocytic THP-1 cells were transfected with siMRP8/14 or siCTL for 24 hours and then challenged with PA. mRNA expression levels of MRP8 (A) and MRP14 (B) as well as pro-inflammatory cytokines, including MIP-2 (C), IL-1β (D), TNF-α (E), and IL-6 (F) were tested using real-time PCR at 6 and 24 hours p.i. Data are the mean ± SEM and represent three individual experiments.
