Figure 7. .

MRP8/14 promoted macrophage-mediated bacterial killing of PA by enhancing ROS, but not NO production. RAW264.7 cells were transfected with siMRP8/14 or siCTL and then challenged with PA. (A) ROS production (as calculated by the percentage of DCF positive cells) was determined by flow cytometry in siMRP8/14- and siCTL-treated cells after PA infection. (C) NO production (as indicated by the nitrite concentration) was tested using the Griess assay in siMRP8/14- and siCTL-treated cells at 6, 18, and 36 hours p.i. mRNA expression levels of NOX2 (B) and iNOS (D) also were tested using real-time PCR at 1 and 2 hours p.i. Data represent three individual experiments.