Figure 2.

Modulation of basal and induced preprotachykinin (PPT) mRNA expression in cultured rat dorsal root ganglion cells by nonsteroidal anti-inflammatory drugs (NSAIDs) and paracetamol. (A) Basal expression of PPT mRNA was significantly reduced by nimesulide but was unchanged by treatment with other NSAIDs or paracetamol (all at 10 μM). (B) Nimesulide, paracetamol, celecoxib, and diclofenac significantly decreased upregulation of PPT mRNA expression induced by the inflammatory soup, while ibuprofen was ineffective.

Notes: The amount of PPT is normalized to glyceraldehyde 3-phosphate dehydrogenase (GAPDH) levels by subtracting the average GAPDH CT value from the average PPT CT value then applying the comparative method (2^−ΔΔCT) using untreated cells as calibrator (controls). All drugs were applied at 10 μM for 36 hours. Values represent means ± standard error of the mean of four to six experiments. *P < 0.05 versus respective controls (analysis of variance followed by Bonferroni’s t-test).