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. Author manuscript; available in PMC: 2014 Feb 15.
Published in final edited form as: Toxicol Appl Pharmacol. 2012 Dec 27;267(1):49–56. doi: 10.1016/j.taap.2012.12.013

Figure 4. Effect of chronic VCD exposure on the GSTM:ASK1 protein complex.

Figure 4

Ovaries from F344 PND4 rats were cultured in control media (CT) or media containing VCD (30 μM) for 4 or 6 d. Ovaries were exposed to VCD on alternate days from the beginning of culture. Following incubation, total protein was isolated and immunoprecipitation was carried out as described in materials and methods. (A) ASK1 immunoprecipitation followed by detection of GSTM protein on d4 or d6; control = c, VCD = v. (B) Quantification of the amount of GSTM protein bound to ASK1. (C) Representative Western blot for unbound GSTM protein on d6. (D) Quantification of the amount of unbound GSTM protein levels on d4 and d6. Values are expressed as a percentage of control; n=3 (20 ovaries per pool). * P < 0.05; different from control.