Figure 5. Effect of acute VCD exposure on GSTM protein level.
Ovaries from F344 PND4 rats were cultured in control (CT) media or media containing VCD (30 μM) for 6d with exposure to VCD at the onset of culture for 2d. Following incubation, total protein was isolated and Western blotting was performed to detect GSTM protein as described in materials and methods. (A) Representative Western blot on d6; control = c, VCD = v. (B) Quantification of GSTM protein levels, values are expressed as a percentage of control; n=3 (10 ovaries per pool). * P < 0.05; different from control.