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F344 PND4 rat ovaries were cultured in media containing control or LY294002 (20 μM) for 4d or 6d. Total protein was isolated and Western blotting was performed to detect GSTM protein levels. (A) Representative Western blot on d6; control = c, LY294002 = LY. (B) Quantification of Western blotting for GSTM protein, values are expressed as a percentage of control; n=3 (20 ovaries per pool). * P < 0.05; different from control.
