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. Author manuscript; available in PMC: 2013 Jun 4.
Published in final edited form as: Nat Commun. 2012;3:1252. doi: 10.1038/ncomms2257

Figure 3. Homomeric TRPP3 complexes on the plasma membrane of Xenopus oocytes contain 3 TRPP3 subunits.

Figure 3

(a) TIRF image from an oocyte expressing TRPP3-EGFP. Immobile spots were selected (circles) for photobleaching analysis. Scale bar, 2 μm. (b) Time course of photobleaching of two representative spots in a, showing three EGFP bleaching steps (arrows). Green bar indicates EGFP excitation. (c) Distribution of observed and calculated bleaching steps for TRPP3-EGFP fluorescence spots. The 1–3 step distribution (green bars) is well fit by a binomial distribution (white bars) that assumes that each spot contains 3 EGFPs and that the probability of EGFP to be fluorescent is 70%.

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