Table 1.
Dimethylamine+ |
Trimethylamine+ |
Mg2+ |
||||
---|---|---|---|---|---|---|
Channel type | ΔErev ± SD (n) | Pdimethylamine/PNa | ΔErev ± SD (n) | Ptrimethylamine/PNa | ΔErev ± SD (n) | PMg/PNa |
Wild Type | −23.6 ± 5.3 (55) | 0.39 | −46.6 ± 8.4 (54) | 0.16 | −5.4 ± 3.7 (49) | 0.65 |
TRPP3_D523Q | −8.1 ± 4.1 (8) | 0.73 | −16.6 ± 8.9 (9) | 0.52 | −23.1 ± 9.1 (8) | 0.27 |
TRPP3_D525K | −12.2 ± 6.9 (11) | 0.62 | −13 ± 4.7 (10) | 0.60 | −12.6 ± 9.1 (9) | 0.45 |
PKD1L3_T2067D | −4.8 ± 1.2 (10) | 0.83 | −16.3 ± 4.6 (8) | 0.53 | 13.2 ± 5.4 (11) | 1.91 |
PKD1L3_T2067P | −2.2 ± 0.8 (7) | 0.92 | −9.7 ± 2.9 (7) | 0.68 | −25.2 ± 4.8 (10) | 0.24 |
PKD1L3_K2069D | −4 ± 0.7 (7) | 0.85 | −13.8 ± 2.2 (7) | 0.58 | 22.6 ± 0.8 (6) | 3.46 |
PKD1L3_E2072A | −26.8 ± 2.7 (7) | 0..35 | −51.4 ± 9 (6) | 0.13 | −15.5 ± 1.2 (7) | 0.39 |
ΔErev is the change of reversal potential upon switching the bath solution from Na+ to the test ions (ΔErev = Erev,X − Erev,Na). Statistical comparisons are given in Fig 1. Results are shown as mean and standard deviation (SD). n = number of measurements.