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Schematic drawing for the construction of strain MUR24Pf. Plasmid pMUR50 possesses a modified chitinase construct together with the resistance cassette as used for the construction of plasmid pMUR47. After transformation with circular pMUR50 the intermediate mutant MUR24Pf_i was isolated and characterized. The negative selection with 6-methylpurine resulted in strain MUR24Pf with a modified version of the chitinase.
